Relative intensities of recognition factors at two combining. We described previously the properties and sequence of the first lectin, rsl subunit m r 9. Engineering of paiil lectin from pseudomonas aeruginosa. Pdf anomerspecific recognition and dynamics in a fucose. In the present study, surface plasmon resonance experiments. Three crystal structures of pail have been reported thus far. Pdf fucosebinding lectin from opportunistic pathogen. Classification and comparison of fucosebinding lectins. Fucosebinding lectins play important roles in many biological processes including cell adhesion, infection of host cells by pathogenic bacteria, and binding to glycosylated proteins. A new type of betapropeller architecture formed by oligomerization and interacting with fucoside, fucosyllactose, and plant xyloglucan.
Another lectin that recognizes oligosaccharides containing core fucose would be a. Ralstonia solanacearum also produces a potent lfuctosebinding lectin, rsl, a small protein of 90 amino acids with a tandem repeat in its amino acid sequence. These lectins may bind to terminal lfucose, darabinose, and. Lectins are the primary recognition macromolecules for various types of fucosylation, a common eukaryotic posttranslational modification. Structures of the lectins from pseudomonas aeruginosa. Aal 10, 11, aspergillus fumigatus afl 12, ralstonia solanacearum rsl, and. Classification and comparison of fucosebinding lectins based. Carbohydratebindingspecificityofafucosespecificlectin from. Ralstonia solanacearum wilts many plants, causing heavy agricultural losses. Structural representation of rsl showing the r2ex data pdf.
The fucosebinding lectin from ralstonia solanacearum. During the past decades, the genome of this microbe relating to the molecular basis of its virulence has been revealed,, including its l fuc binding lectin. Ralstonia solanacearum strain gmi pseudomonas solanacearum status. The trimeric solution structure and fucosebinding mechanism of the. Many threedimensional structures of fucosebinding lectins have been reported. Heterologous expression and molecular binding properties. Pdf production, properties and specificity of a new. In the present study, surface plasmon resonance experiments conducted on a series of. This is the first reported fucosespecific lectin found in nematophagous fungi. Files available from the acs website may be downloaded for personal use only. Ralstonia solanacearum is one of the most destructive phytopathogenic bacteria on earth causing great agricultural and economical losses. In silico mutagenesis and docking study of ralstonia. With the exception of three lone proteins, these lectins can be classified into six families.
Jul 29, 2005 the bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent l fucose binding lectin, r. The bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent l fucose binding lectin, r. The bacterium ralstonia solanacearum, which is distributed worldwide and causes lethal wilt in many agricultural crops, was shown to produce a potent lfucosebinding lectin, r. Feb 03, 2012 analysis of the available genomes of b. Six independent fucosebinding sites in the crystal structure of.
Anomerspecific recognition and dynamics in a fucosebinding. As a result, the lectin protein has an high affinity for the. We compiled and compared the fucose binding lectins whose structures have been solved to date. Rsl has a trimetric structure mw 30 kda composed of 90 amino acids 10.
The crystal structure of aol a fucosespecific lectin of aspergillus oryzae has. The present communication reports the discovery of the second one, rs. The plant pathogen ralstonia solanacearum produces two lectins, each with different affinity to fucose. Kostlanova n1, mitchell ep, lortatjacob h, oscarson s, lahmann m, gilboagarber n, chambat g, wimmerova m, imberty a. In this study, we report the heterologous expression and molecular binding properties of a fucosespecific lectin, aoflea, isolated from arthrobotrys oligospora.
Ralstonia solanacearum strain gmi pseudomonas solanacearum. A typical lectin free diet excludes a range of foods, including most grains, pulses, and legumes, as well as eggs, seafood, and many staple fruits and vegetables. Carbohydratebindingspecificityofafucosespecificlectin. We described previously the properties and sequence of the first lectin, rsl subunit mr 9. Plant pathogens, like animal ones, use proteincarbohydrate interactions in their strategy for host recognition, attachment, and invasion. In all cases, the quaternary structure is a tetramer arranged around a pseudo c222 axis of symmetry. Fucosebinding lectin from opportunistic pathogen burkholderia. The worldwide distributed plant aggressive pathogen ralstonia solanacearum, which causes lethal wilt in many agricultural crops, produces a potent lfucosebinding lectin rsl exhibiting sugar. The lectin pail 51 kda, composed of four subunits of 121 amino acids, binds dgalactose plus its derivatives, and the lectin paiil 47 kda, composed of four subunits of 114 amino acids, binds lfucose and other monosaccharides.
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